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Proteintech il 18
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R&D Systems quantikine human total mmps immunoassay
(A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The <t>Quantikine</t> Human Total <t>MMPs</t> <t>Immunoassay</t> (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.
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AbCys s a human il-12 total elisa, abc 261/2
(A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The <t>Quantikine</t> Human Total <t>MMPs</t> <t>Immunoassay</t> (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.
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Bio-Techne corporation human il-18/il-1f4 elisa kit (colorimetric)
(A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The <t>Quantikine</t> Human Total <t>MMPs</t> <t>Immunoassay</t> (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.
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Image Search Results


(A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.

Journal: PLoS ONE

Article Title: Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression

doi: 10.1371/journal.pone.0124865

Figure Lengend Snippet: (A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP1, MMP2, MMP3, MMP9, and TIMP1 in the cell culture supernatants.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, MANN-WHITNEY